Abstract
WHEN ram spermatozoa are chilled and frozen, their acrosomes are damaged even in the presence of protective substances in the diluent. It has been suggested1 that these structural changes contribute to the reduced fertility of frozen ram semen. Although the motility and structure of spermatozoa are affected to different extents by freezing it is not known if these changes occur simultaneously or are caused at different stages of the freezing process.
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References
Watson, P. F., and Martin, I. C. A., J. Reprod. Fert., 28, 99–101 (1972).
Emmens, C. W., J. Physiol., Lond., 106, 471–481 (1947).
Bank, H., and Mazur, P., Expl Cell Res., 71, 441–454 (1972).
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WATSON, P., MARTIN, I. Regions of the freezing curve causing changes in structure and viability of ram sperm. Nature 251, 315–316 (1974). https://doi.org/10.1038/251315a0
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DOI: https://doi.org/10.1038/251315a0
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