Abstract
THE immune response following grafting of allogeneic or semiallogeneic cells in the mouse is characterised by the development of cytotoxic effector lymphocytes and/or antibodies which become cytotoxic in vitro in the presence of complement. I have studied the kinetics of the humoral response in DBA/2 inbred mice injected with semiallogeneic somatic cell hybrids and shown that these hybrid cells were unexpectedly resistant to the complement-dependent cytotoxic action of antisera from the initial peak of the biphasic response. The allogeneic parental cells were however highly sensitive to cytolysis under the same experimental conditions. This serological response seems to be directed against the H-2 alloantigens expressed on both the allogeneic parental cells and on the semiallogeneic hybrid cells derived from it. In my attempts to understand this phenomenon, I found that the mouse alloantibodies of the IgM and IgG classes have different efficiencies when tested in the cytotoxic test on cells having a low H-2 antigen concentration on their plasma membrane.
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RUBIO, N. Surface H-2 antigen concentration requirement of somatic hybrid cells for IgM-mediated cytotoxicity. Nature 249, 461–463 (1974). https://doi.org/10.1038/249461a0
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DOI: https://doi.org/10.1038/249461a0
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