Abstract
RECENT studies suggest that processing of ribosomal precursor RNA might be impaired in blast cells of acute leukaemia (AML)1,2. Following incubation with 3H-methyl-methionine blast cells of human acute leukaemias partially failed to methylate the 45S and 32S ribosomal precursors1. Using autoradiographic techniques, previous studies have also shown an increase in intranuclear RNA and decreased cytoplasmic RNA in these cells3. More recently, competitive hybridisation was performed with normal lymphocyte DNA and 35S–55S RNA fractions extracted from both acute leukaemia blast cells and normal PHA-stimulated lymphocytes. Using incubations with actinomycin D these experiments have led to the conclusion that leukaemic blast cells synthesise heterogeneous nuclear RNA molecules which are not transcribed in PHA-stimulated lymphocytes and it was also suggested—in contrast to the earlier data—that the amount of unmethylated unprocessed rRNA precursor was small in leukaemic blast cells4.
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References
Torelli, U. L., Torelli, G. M., Andreoli, A., and Mauri, C., Nature, 226, 1163 (1970).
Torelli, U. L., Torelli, G. M., Andreoli, A., and Mauri, C., Acta haemat., 45, 201 (1971).
Hyman, G. A., Fingerhut, B., and Tiburcio, A. C., Cancer, 21, 357 (1968).
Torelli, U. L., Torelli, G. M., and Mauri, C., Eur. J. Cancer, 8, 653 (1972).
Seeber, S., and Schmidt, C. G., Klin. Wschr., 51, 677 (1973).
Dounce, A. L., in The Nucleic Acids (edit by Chargaff, E. and Davidson, J. N.), 2, 93 (Academic Press, New York, 1955).
Scherrer, K., and Darnell, J. E., Biochem. biophys. Res. Commun., 7, 468 (1962).
Seeber, S., and Busch, H., Cancer Res., 31, 1888 (1971).
Attardi, G., and Amaldi, F., A. Rev. Biochem., 39, 183 (1970).
Cooper, H. L., J. biol. Chem., 243, 34 (1968).
Seeber, S., Choi, Y. C., and Busch, H., J. biol. Chem., 246, 2633 (1971).
Seeber, S., and Busch, H., J. biol. Chem., 246, 7151 (1971).
Rubin, A. D., and Cooper, H. L., Proc. natn. Acad. Sci. U.S.A., 54, 469 (1965).
Cooper, H. L., J. biol. Chem., 244, 1946 (1969).
Rubin, A. D., J. clin. Invest., 50, 2485 (1971).
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SEEBER, S., KÄDING, J., BRUCKSCH, K. et al. Defective rRNA synthesis in human leukaemic blast cells?. Nature 248, 673–675 (1974). https://doi.org/10.1038/248673a0
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DOI: https://doi.org/10.1038/248673a0
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