Abstract
CULTURED cells of both animal and plant tissues are characterised by instability of chromosome number and structure1,2. Although there is evidence3 that polyploid plant cell lines may arise from endoreduplicated nuclei in the original explant, the range of chromosome number and structure observed in established cultures strongly points to the origin of these changes during culture. Since the precise distribution of chromosomes at mitotic anaphase is the essential prerequisite for chromosome number stability5, this seems the most likely point at which instability could be induced in culture. It has been shown6,7 that, in culture, animal cells of various species are characterised by the presence of multipolar mitoses, a feature which would produce daughter nuclei with aneuploid chromosome numbers. Such multipolar mitoses have been briefly noted for plant tissue cultures8,9 but their frequencies have not been quantified.
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References
Sunderland, N., in Plant Tissue and Cell Culture (edit. by Street, H. E.), chap. 7 (Blackwell, Oxford, 1973).
Harris, M., Cell Culture and Somatic Variation (Holt, Rinehart and Winston, New York and London, 1964).
Torrey, J. G., Proc. int. Conf. Plant Tissue Cult. (edit. by Grove, P. R., and White, A. R.), 473 (McCutchain, Berkeley, 1965).
Kao, K. N., Miller, R. A., Gamborg, O. L., and Harvey, B. L., Can. J. Genet. Cytol., 12, 297 (1970).
Luykx, P., Int. Rev. Cytol. Suppl II (1970).
Pera, F., and Schwarzacher, A. G., Chromosoma, 26, 337 (1969).
Fetner, R. H., and Porter, E. D., Expl Cell Res., 37, 429 (1965).
Straus, J., Am. J. Bot., 41, 833 (1954).
Singh, B. D., Harvey, B. L., Kao, K. N., and Miller, R. A., Can. J. Genet. Cytol., 14, 65 (1972).
Smith, S. M., and Street, H. E., Annls Bot. (in the press).
McWilliam, A., Smith, S. M., and Street, H. E., Annls Bot. (in the press).
Croker, B. H., Bot. Gaz., 114, 274 (1953).
Mohandas, T., and Grant, W. F., Can. J. Genet. Cytol., 14, 773 (1972).
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BAYLISS, M. Origin of Chromosome Number Variation in Cultured Plant Cells. Nature 246, 529–530 (1973). https://doi.org/10.1038/246529a0
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DOI: https://doi.org/10.1038/246529a0
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