Abstract
IT is often difficult to obtain highly radioactive in vivo 32P-labelled RNAs from higher organisms for fingerprinting1. In such situations, in vitro labelling methods can make a valuable contribution. One method using polynucleotide phosphokinase to introduce 32P at the 5′ terminus of oligonucleotides has been reported2,3. Here I describe another method which is based on the fact that iodine has a high specificity for cytosine under certain conditions4 and that iodine isotopes (125I, 131I) are easily detectable by autoradiography. I have used Escherichia coli formyl-methionine tRNA of known sequence5,6 to test the suitability of this method for fingerprinting RNAs. The results show that a fingerprint of pmol amounts of RNA is obtainable within 2 d.
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DUBE, S. Use of 125I in Fingerprinting RNA. Nature 246, 483 (1973). https://doi.org/10.1038/246483a0
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DOI: https://doi.org/10.1038/246483a0
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