Abstract
MANY plant tissue cultures change in growth rate, chromosome cytology and morphogenic potential during repeated subculture1–3. Controlled freezing and low temperature storage of cultured plant cells might enable the characters of newly initiated cultures to be preserved. Cell preservation at the temperature of liquid N2 (−196° C) has been successful with animal cells4 and preliminary work with plant cells has shown the promise of this approach5–7. Latta has reported regrowth of cultures of carrot (Daucus carota L.) following freezing for up to 2 months at −40° C or at −196° C but did not assess the percentage of cells surviving6.
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References
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NAG, K., STREET, H. Carrot Embryogenesis from Frozen Cultured Cells. Nature 245, 270–272 (1973). https://doi.org/10.1038/245270a0
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DOI: https://doi.org/10.1038/245270a0
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