Abstract
AFTER the recognition of “capacitation of sperm” by Austin1 and Chang2, successful in vitro fertilization of mammalian eggs was achieved in many species3–17. Although fertilization of rat eggs in vitro has been attempted1,18–20, incorporation of sperm into the vitellus was observed only after the dissolution of zona pellucida by chymotrypsin19. Here we report fertilization of intact rat eggs in vitro and the results obtained.
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References
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MIYAMOTO, H., CHANG, M. In vitro Fertilization of Rat Eggs. Nature 241, 50–52 (1973). https://doi.org/10.1038/241050a0
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DOI: https://doi.org/10.1038/241050a0
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