Abstract
SELECTION and propagation of a single antibody-forming cell clone has been effected by serial transfer of limited numbers of spleen cells into irradiated syngeneic mice1. Original spleen cell donors were immunized with DNP conjugated to bovine gamma globulin (BGG). A single clone was identified by its characteristic secreted antibody to DNP. Sera of recipient mice were analysed by isoelectric focusing on polyacrylamide gels, and specific antibody molecules were visualized by 131I-hapten binding and autoradiography2. Using the antibody isoelectric spectrum as a phenotypic marker, we were able to follow one clone of anti-DNP forming cells (E9) through successive transfer generations; at the time of our first report1, clone E9 had been propagated through five transplant generations.
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WILLIAMSON, A., ASKONAS, B. Senescence of an Antibody-forming Cell Clone. Nature 238, 337–339 (1972). https://doi.org/10.1038/238337a0
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DOI: https://doi.org/10.1038/238337a0
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