Abstract
SEVERAL investigators have reported culturing two and four-celled mammalian ova to the blastocyst stage1–5. Although successful in vitro cultivation of eggs from the single cell to the blastocyst stage has been achieved in the mouse6–8, few satisfactory methods have been devised for the culture of the ova, at this stage, of other mammalian species, apart from some experiments with human oocytes9–10. We now wish to report a higher rate of success with one-celled rabbit ova cultivated in vitro to the blastocyst stage, using a nutrient solution composed of several known synthetic media.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Brinster, R. L., Exp. Cell Res., 32, 205 (1963).
Brinster, R. L., J. Reprod. Fert., 10, 227 (1965).
Biggers, J. D., Moore, B. D., and Whittingham, D. G., Nature, 206, 734 (1965).
Onuma, H., Maurer, R. R., and Foote, R. H., J. Reprod. Fert., 16, 491 (1968).
Kane, M. T., and Foote, R. H., Biol. Reprod., 2, 245 (1970).
Biggers, J. D., Gwatkin, R. B. L., and Brinster, R. L., Nature, 194, 747 (1962).
Whitten, W. K., and Biggers, J. D., J. Reprod. Fert., 17, 399 (1968).
Mukherjee, A. B., and Cohen, M. M., Nature, 228, 472 (1970).
Edwards, R. G., Steptoe, P. C., and Purdy, J. M., Nature, 227, 1307 (1970).
Steptoe, P. C., Edwards, R. G., and Purdy, J. M., Nature, 229, 132 (1971).
Ham, R. G., Proc. US Nat. Acad. Sci., 53, 288 (1965).
Moore, G. G., Gerner, R. E., and Addison, H. F., J. Amer. Med. Assoc., 199, 519 (1967).
Eagle, H., Science, 130, 432 (1959).
Kuhl, W., and Friedrich-Freksa, H., Zool. Anz., Suppl., 9, 187 (1936).
Borghese, E., and Cassini, A., in Cinemicrography in Cell Biology (edit. by Rose, G. G.), 274 (Academic Press, New York, 1963).
Cole, R. J., and Paule, J., Preimplantation Stages of Pregnancy, Ciba Foundation Symp. (edit. by Wolstenholme, G. E. W., and O'Conner, M.), 82 (Little Brown, Boston, 1965).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
OGAWA, S., SATOH, K. & HASHIMOTO, H. In vitro Culture of Rabbit Ova from the Single Cell to the Blastocyst Stage. Nature 233, 422–424 (1971). https://doi.org/10.1038/233422a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/233422a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
