Abstract
UNSCHEDULED synthesis of DNA has been observed in the nuclei of HeLa cells at some stage of the cell cycle other than the S-phase when cells are exposed in vitro to ultraviolet light or X-ray irradiation1. It has since been confirmed for several other cell types2–5. Part of the synthesis defined in terms of the uptake of 3H-thymidine—which was detected in HeLa cells as the result of large doses of ultraviolet irradiation—was non-semiconservative (repair replication), corresponding to the insertion of DNA precursors into extant parental DNA strands. Here I present evidence that X-irradiation of a 1 day old female guinea-pig with 5,000 rad is followed by a small but auto-radiographically detectable incorporation of 3H-thymidine into the DNA of diplotene oocytes. In the guinea-pig, the mouse and most other mammals the premeiotic DNA synthesis occurs during embryonic life6. After birth the oocytes have all entered prophase of meiosis during which no DNA synthesis is normally observed.
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CRONE, M. Radiation Stimulated Incorporation of 3H-Thymidine into Diplotene Oocytes of the Guinea-pig. Nature 228, 460 (1970). https://doi.org/10.1038/228460a0
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DOI: https://doi.org/10.1038/228460a0
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