Abstract
THE lac represser binds very specifically and tightly to the lac operator1–3. In conditions where the repressor binds to operator with a dissociation constant of 10−13 M, the binding of represser to DNA not containing the Escherichia coli lac operator (non-operator DNA) was less tight by several orders of magnitude3. Although weak, we thought this non-operator DNA binding could be highly significant and deserved careful study. We report here on the binding of lac represser to non-operator DNA from several natural sources and also to synthetic poly dAT.
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References
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LIN, SY., RIGGS, A. Lac Repressor Binding to DNA not containing the Lac Operator and to Synthetic Poly dAT. Nature 228, 1184–1186 (1970). https://doi.org/10.1038/2281184a0
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DOI: https://doi.org/10.1038/2281184a0
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