Abstract
SUBCELLULAR fractions of bulked protein bodies isolated from the storage tissues of seeds contain several or many types of protein1–3, but in general it is not known whether this diversity occurs within an individual protein body, or whether the different proteins are deposited in separate compartments during seed maturation. Immunofluorescence techniques have enabled us to resolve this question in respect of the subcellular distribution of legumin and vicilin, two important globulins stored in the cotyledons of many legumes4–6.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Varner, J. E., and Schidlovsky, G., Plant. Physiol., 38, 139 (1963).
Briarty, L. G., thesis, Univ. Liverpool (1967).
Catsimpoolas, N., Campbell, T. G., and Meyer, E. W., Plant Physiol., 43, 799 (1968).
Danielsson, C.-E., Biochem. J., 44, 387 (1949).
Kloz, J., and Turkova, V., Biol. Plant., 5, 29 (1963).
Jackson, P., Boulter, D., and Thurman, D. A., New Phytol., 68, 25 (1969).
Avrameas, S., and Ternynck, T., Immunochemistry, 6, 53 (1969).
Goldman, M., Fluorescent Antibody Methods (Academic Press, New York and London, 1968).
Danielsson, C.-E., Acta Chem. Scand., 3, 41 (1949).
Vaintraub, I. A., Shutov, A. D., and Klimenko, V. G., Biochemistry (USSR), 27, 289 (1962).
Gunning, B. E. S., and Pate, J. S., Protoplasma, 68, 107 (1969).
Briarty, L. G., Coult, D. A., and Boulter, D., J. Exp. Bot., 20, 358 (1969).
Mazia, D., Brewer, P. A., and Alfert, M., Biol. Bull., 104, 56 (1953).
Robinow, C. F., and Marak, J., J. Cell Biol., 29, 129 (1966).
Flinn, A. M., and Pate, J. S., Ann. Bot., 32, 479 (1968).
Kloz, J., Turkova, V., and Klozova, E., Biol. Plant., 8, 164 (1966).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
GRAHAM, T., GUNNING, B. Localization of Legumin and Vicilin in Bean Cotyledon Cells using Fluorescent Antibodies. Nature 228, 81–82 (1970). https://doi.org/10.1038/228081a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/228081a0
This article is cited by
-
Expression profiles of miRNAs in the genesis of Populus euphratica Oliv. heteromorphic leaves
Plant Growth Regulation (2017)
-
Immunolocalization of chromosome-associated proteins in plants – principles and applications
Botanical Studies (2014)
-
Localization of mRNA for pea legumin:In situ hybridization using a biotinylated cDNA probe
Protoplasma (1986)
-
The major albumin protein from pea (Pisum sativum L.)
Planta (1985)
-
Periodate-acid treatment of sections permits on-grid immunogold localization of pea seed vicilin in ER and Golgi
Protoplasma (1984)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
