Abstract
WE have found that when the ribosomes of Escherichia coli Q13 are treated with chloramphenicol (CM) for a long time they become deficient in protein and also defective in MS2 RNA directed in vitro protein synthesis. On removal of CM, for 90 min or longer, there was a lag before the rate of protein synthesis recovered. For example, a lag period of about 180 min was observed after 180 min with CM. It seems that CM has an effect besides its direct inhibitory action on peptide formation1. It may cause alterations in ribosomes which result in a delayed resumption of protein synthesis.
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YOUNG, R., NAKADA, D. Protein-deficient Ribosomes in Chloramphenicol-treated Escherichia coli. Nature 227, 604–606 (1970). https://doi.org/10.1038/227604a0
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DOI: https://doi.org/10.1038/227604a0
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