Abstract
WE have been able to stimulate the formation in rabbits of a very specific antibody against triiodothyronine (T3) by injecting the animals with a condensation product of T3 and succinyl poly-L-lysine. Antibodies against thyroxine (T4) have been obtained by Clutton, Harington and Yuill1 and by Churchill and Tapley2 using conjugated hapten–protein antigens, but the use of natural proteins as carriers may be the reason for the rather low specificity of the resulting antibodies. We prepared succinylated poly-L-lysine by the method of Stason, Vallotton and Haber3, and reacted it by carbodiimide condensation with the methyl ester of T3. After saponification a highly specific antigen was obtained.
Similar content being viewed by others
Article PDF
References
Clutton, R. F., Harington, C. R., and Yuill, M. E., Biochem. J., 32, 1119 (1938).
Churchill, W. H., and Tapley, D. F., Nature, 202, 29 (1964).
Stason, W. B., Vallotton, M., and Haber, E., Biochim. Biophys. Acta, 133, 582 (1967).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
BROWN, B., EKINS, R., ELLIS, S. et al. Specific Antibodies to Triiodothyronine Hormone. Nature 226, 359 (1970). https://doi.org/10.1038/226359a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/226359a0
This article is cited by
-
Poly(ADP-ribosyl)ation of proteins and germ cell development in hyperthyroid rat testes
Molecular and Cellular Biochemistry (2009)
-
Thyroid state and mitochondrial population during cold exposure
PflĂĽgers Archiv (1983)
-
Thyroid state and mitochondrial population during maturation and ageing
Journal of Endocrinological Investigation (1980)
-
Specificity of antibodies to arginine-vasopressin raised with succinylated poly-l-lysine as carrier
Experientia (1971)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.