Abstract
THE recognition of its tRNA substrate by an aminoacyl-tRNA synthetase is an essential step in the translation of genetic information into amino-acid sequences of proteins. An understanding of this recognition requires a knowledge of the structure of the enzyme. Several aminoacyl-tRNA synthetases have been prepared in highly purified form, but they have so far not been obtained in a crystalline form suitable for crystallographic analysis1–3. We report the crystallization of a lysyl-tRNA synthetase purified from yeast.
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Davie, E. W., Koningsberger, V. V., and Lipmann, F., Arch. Biochem. Biophys., 65, 21 (1956).
Makman, M. H., and Cantoni, G. L., Biochemistry, 4, 1434 (1965).
Preddie, E. C., J. Biol. Chem., 277, 3958 (1969).
Lagerkvist, U., Rymo, L., and Waldenström, J., J. Biol. Chem., 271, 5391 (1966).
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RYMO, L., LAGERKVIST, U. Crystallization of Lysyl-tRNA Synthetase from Yeast. Nature 226, 77 (1970). https://doi.org/10.1038/226077a0
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DOI: https://doi.org/10.1038/226077a0
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