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Mild “Unstable Haemoglobin Haemolytic Anaemia” caused by Haemoglobin Shepherds Bush (B74 (E18) Gly→Asp)

Nature volume 225, pages 939–941 (1970)Cite this article

Abstract

A 30 YEAR old South African woman of British extraction was investigated because of a persistent reticulocytosis. As a child she had been chronically anaemic, and at the age of six her spleen had been removed. After that time she had been well, but on one occasion she became “ill” after a course of sulphonamide. She was found to have a well compensated haemolytic anaemia; the haemoglobin level was 13 g/100 ml. of blood, and 5–8 per cent of her red cells were reticulocytes. Though of normal size the red cells showed target cell formation, basophilic stippling, and 75–80 per cent contained Heinz bodies. The test for abnormally heat labile haemoglobin1 was positive. The patient's mother was found to carry the same haemoglobin abnormality but was not thought to suffer from severe haemolysis. She did, however, experience an episode of severe haemoglobinuria following sulphonamides.

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References

  1. Dacie, J. V., Grimes, A. J., Meisler, A., Steingold, L., Hemsted, E. H., Beaven, G. H., and White, J. C., Brit. J. Haematol., 10, 388 (1964).

    Article  CAS  Google Scholar 

  2. Betke, K., Marti, H. R., and Schlicht, I., Nature, 184, 1877 (1959).

    Article  ADS  CAS  Google Scholar 

  3. Cradock-Watson, J. E., Fenton, J. C. B., and Lehmann, H., J. Clin. Pathol., 12, 372 (1959).

    Article  CAS  Google Scholar 

  4. Poulik, M. D., Nature, 180, 1477 (1957).

    Article  ADS  CAS  Google Scholar 

  5. Huisman, T. H. J., and Dozy, A. M., J. Chromatog., 19, 160 (1965).

    Article  CAS  Google Scholar 

  6. Chernoff, A. I., and Pettit, N. M., Blood, 24, 750 (1965).

    Google Scholar 

  7. Clegg, J. B., Naughton, M. A., and Weatherall, D. J., J. Mol. Biol., 19, 91 (1966).

    Article  CAS  Google Scholar 

  8. Dacie, J. V., Shinton, N. K., Gaffney, P. J., Carrell, R. W., and Lehmann, H., Nature, 216, 663 (1967).

    Article  ADS  CAS  Google Scholar 

  9. Sick, K., Beale, D., Irvine, D., Lehmann, H., Goodall, P. T., and MacDougall, S., Biochim. Biophys. Acta, 140, 231 (1967).

    Article  CAS  Google Scholar 

  10. Lehmann, H., and Huntsman, R. G., Man's Haemoglobins, 299 (North-Holland, Amsterdam, 1966).

    Google Scholar 

  11. Smith, I., Chromatographic and Electrophoretic Techniques, 1 (Heinemann, London, 1969).

    Google Scholar 

  12. Beale, D., Biochem. J., 103, 129 (1967).

    Article  CAS  Google Scholar 

  13. Nirenberg, M., Leder, P., Bernfield, M., Brimacombe, R., Trupin, J. Rotman, F., and O'Neal, C., Proc. US Nat. Acad. Sci., 53, 1161 (1965).

    Article  ADS  CAS  Google Scholar 

  14. Ambler, R. P., and Meadway, R. J., Biochem. J., 108, 893 (1968).

    Article  CAS  Google Scholar 

  15. Matsubara, H., Biochem. Biophys. Res. Commun., 24, 427 (1969).

    Article  Google Scholar 

  16. Matsubara, H., Singer, A., and Saski, R. M., Biochem. Biophys. Res. Commun., 34, 719 (1969).

    Article  CAS  Google Scholar 

  17. Perutz, M. F., J. Mol. Biol., 13, 646 (1965).

    Article  CAS  Google Scholar 

  18. Perutz, M. F., Kendrew, J. C., and Watson, H. C., J. Mol. Biol., 13, 669 (1965).

    Article  CAS  Google Scholar 

  19. Perutz, M. F., Muirhead, M., Cox, J. M., and Goodman, L. C. G., Nature, 219, 131 (1968).

    Article  ADS  CAS  Google Scholar 

  20. Dayhoff, M. O., and Eck, R. V., Atlas of Protein Sequence and Structure (Biochemical Research Foundation, Silverspring, Maryland, 1968).

    Google Scholar 

  21. Hill, R. L., Harris, C. M., Naylor, J. F., and Sams, W. M., J. Biol. Chem., 244, 2182 (1969).

    CAS  PubMed  Google Scholar 

  22. Bradshaw, R. A., and Gurd, F. N., J. Biol. Chem., 244, 2167 (1969).

    CAS  PubMed  Google Scholar 

  23. Guzzo, A. V., Biophys. J., 5, 809 (1965).

    Article  ADS  CAS  Google Scholar 

  24. Perutz, M. F., and Lehmann, H., Nature, 219, 902 (1968).

    Article  ADS  CAS  Google Scholar 

  25. Hollender, A., Lorkin, P. A., Lehmann, H., and Svensson, N., Nature, 222, 935 (1969).

    Article  Google Scholar 

  26. Perutz, M. F., Proc. Roy. Soc., B, 173, 113 (1969).

    ADS  CAS  Google Scholar 

Download references

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Author notes

  1. J. M. WHITE and M. C. BRAIN: MRC Research Group on Haemolytic Mechanisms, Royal Postgraduate Medical School, London.

  2. P. A. LORKIN and H. LEHMANN: MRC Abnormal Haemoglobin Unit, Department of Biochemistry, University of Cambridge.

Authors and Affiliations

  1. Galton Laboratory, University College, London

    J. M. WHITE, M. C. BRAIN, P. A. LORKIN, H. LEHMANN & M. SMITH

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  4. H. LEHMANN
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WHITE, J., BRAIN, M., LORKIN, P. et al. Mild “Unstable Haemoglobin Haemolytic Anaemia” caused by Haemoglobin Shepherds Bush (B74 (E18) Gly→Asp). Nature 225, 939–941 (1970). https://doi.org/10.1038/225939a0

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