Abstract
THE isolation from mammalian brain of a protein with properties similar to those of muscle actomyosin was recently reported1 and it was suggested that the protein was composed of actin-like and myosin-like subunits. There have also been references2 to the possibility that colchicine binding protein isolated from the microtubules of sea urchin sperm tails3, mitotic spindles4,5 and the cilia of Tetrahymena6 may be composed of an actin-like protein. The ability to bind colchicine also seems to be a characteristic of the microtubules of the central nervous system7 and a colchicine binding protein has been isolated from mammalian brain8. It was therefore of interest to isolate colchicine binding protein from brain and test its actin-like properties.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Puszkin, S., Berl, S., Puszkin, E., and Clarke, D. D., Science, 161, 170 (1968).
Schmitt, F. O., and Samson, jun., F. E., Neurosci. Res. Program Bull., 6, 117 (1968).
Shelanski, M. L., and Taylor, E. W., J. Cell Biol., 34, 549 (1967).
Sakai, H., Biochim. Biophys. Acta, 112, 132 (1966).
Kiefer, B., Sakai, H., Solari, A. J., and Mazia, D., J. Mol. Biol., 20, 75 (1966).
Renaud, F. L., Rowe, A. J., and Gibbons, I. R., J. Cell Biol., 36, 79 (1968).
Borisy, G. G., and Taylor, E. W., J. Cell Biol., 34, 525 (1967).
Weisenberg, R. C., Borisy, G. G., and Taylor, E. W., Biochemistry, 7, 4466 (1968).
Asai, H., Biochemistry, 2, 458 (1963).
Richards, E. G., Chung, C.-S., Menzel, D. B., and Olcott, H. S., Biochemistry, 6, 528 (1967).
Marsh, B. B., Biochim. Biophys. Acta, 32, 357 (1959).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
PUSZKIN, S., BERL, S. Actin-like Properties of Colchicine Binding Protein isolated from Brain. Nature 225, 558–559 (1970). https://doi.org/10.1038/225558a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/225558a0
This article is cited by
-
Biophysical characterization of peyer’s patch lymphocytes in the B-cell deficient CBA/N mice and in their F1 hybrids
Cell Biophysics (1979)
-
Electrically-induced mechanical activity of the isolated guinea-pig sciatic nerve. Influences of calcium and anoxia
Experientia (1977)
-
Actin–tubulin homology revisited
Nature (1975)
-
Axon retraction following guanethidine treatment: studies of sympathetic neurons in tissue culture
Journal of Neurocytology (1974)
-
Axon retraction following guanethidine treatment
Zeitschrift für Zellforschung und Mikroskopische Anatomie (1973)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
