Abstract
RECONSTITUTED ribosomes which lack some ribosomal proteins were found to be inactive in catalysing poly-U-dependent phenylalanine incorporation1. A similar result was previously reported resulting from an alteration of the 50S ribosomal subunit by a temperature sensitive mutation2. Temperature sensitive mutants of Escherichia coli K12 defective in protein synthesis have been isolated in this laboratory, and I report here, for the first time, that mutant strain C1716 was much less active in poly-U-dependent polyphenylalanine synthesis due to an alteration of the 30S ribosomal subunit.
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KANG, SS. Temperature Sensitive Alteration of 30S Subunits demonstrated by in vitro Reassociation of Functional Ribosomes. Nature 225, 1132–1133 (1970). https://doi.org/10.1038/2251132a0
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DOI: https://doi.org/10.1038/2251132a0
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