Abstract
Sylvén and Snellman1 have reported that highly purified beef spleen cathepsin B catalyses the hydrolysis of both benzoylarginine β-naphthylamide (pH. 6.0 optimum) and leucine β-naphthylamide (pH 7.3 optimum). The latter effect was termed “leucine naphthylamidase” activity. Action on both substrates was attributed to a single enzyme, namely, cathepsin B. Maximal rates of hydrolysis were obtained only when cysteine and EDTA were included in the reaction mixtures, a finding that is consistent with the needs of cathepsin B (ref. 2).
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Sylvén, B., and Snellman, O., Histochemie, 12, 240 (1968).
Greenbaum, L. M., and Fruton, J. S., J. Biol. Chem., 226, 173 (1957).
Niemi, M., and Sylvén, B., Histochemie, 18, 40 (1969).
Chayen, J., Bitensky, L., Butcher, R. G., and Poulter, L. W., Nature, 222, 281 (1969).
Ellis, S., and Perry, M., Biochem. Biophys. Res. Commun., 15, 502 (1964).
Ellis, S., and Perry, M., J. Biol. Chem., 241, 3679 (1966).
Otto, K., Z. Physiol. Chem., 348, 1449 (1967).
Otto, K., and Schepers, P., Z. Physiol. Chem., 348, 482 (1967).
Sawant, P. L., Shibko, S., Kumta, V. S., and Tappel, A. L., Biochim. Biophys. Acta, 85, 82 (1964).
Snellman, O., Biochem. J., 114, 673 (1969).
McDonald, J. K., Zeitman, B. B., Reilly, T. J., and Ellis, S., J. Biol. Chem., 244, 2693 (1969).
McDonald, J. K., Ellis, S., and Reilly, T. J., J. Biol. Chem., 241, 1494 (1966).
Barrett, A. J., and Poole, A. R., Nature, 244, 279 (1969).
Dingle, J. T., and Barrett, A. J., Biochem. J., 105, 19p (1867).
Barrett, A. J., and Dingle, J. T., Biochem. J., 105, 20p (1967).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MCDONALD, J., ZEITMAN, B. & ELLIS, S. Leucine Naphthylamide: an Appropriate Substrate for the Histochemical Detection of Cathepsins B and B′. Nature 225, 1048–1049 (1970). https://doi.org/10.1038/2251048a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/2251048a0
This article is cited by
-
Human skin proteases
Archives for Dermatological Research (1976)
-
Histochemical evidence of cell death in transplanted tumours
Virchows Archiv B Cell Pathology (1972)
-
Hormonabhängige Enzymverteilung in Geweben
Histochemie (1971)
-
Subcellular Distribution of Neutral Protease and Peptidases in Rabbit Polymorphonuclear Leucocytes
Nature (1970)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
