Abstract
ONE of the limiting factors in attempts to set up cell-free systems for the manufacture of endogenous proteins is the preservation of intact polysomes. The strand of messenger RNA connecting the bacterial ribosomes is very fragile and is easily broken by mechanical cell disruption1–4. In consequence the commonly used procedures for disrupting bacterial cells result in extensive degradation.
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GOULD, H., HERBERT, B. & LOVINY, T. Polysomes from Bacillus subtilis and Bacillus thuringiensis. Nature 223, 855–857 (1969). https://doi.org/10.1038/223855a0
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DOI: https://doi.org/10.1038/223855a0
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