Abstract
ALTHOUGH a considerable amount of work has been carried out on the cultivation in vitro of mammalian ova, little has been said about embryonic development after the blastocyst stage. Cole and Paul1, discussing the properties of cultured, preimplantation rabbit embryos, said they observed beating myocardial cells, muscle cell types, blood islands and nerve cells. They mentioned that if the zona pellucida were removed using pronase, an embryo would form, the amniotic folds would close and the beating heart would develop. No details or data were given about the development of the heart beat. Our purpose is to describe the successful cultivation in vitro of rabbit blastocysts which have developed to pulsating embryos.
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References
Cole, R. J., and Paul, J., Ciba Found. Symp. Preimplantation Stages of Pregnancy (edit. by Wolstenholme, G. E. W., and O'Conner, M.), 82 (Little, Brown and Co., Boston, 1965).
Baluda, M. A., and Goetz, I. E., Virology, 15, 185 (1961).
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OGAWA, S., IMAGAWA, D. Development of Pulsating Embryos from Rabbit Blastocysts cultivated in vitro. Nature 223, 409–410 (1969). https://doi.org/10.1038/223409a0
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DOI: https://doi.org/10.1038/223409a0
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