Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Synthesis of Rabbit Globin by a Bacterial Cell Free System

Abstract

A cell free system from E. coli primed with an RNA preparation from rabbit reticulocytes produces a material having properties of rabbit globin. The presence of N-acetyl-valyl-tRNA seems essential.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Chantrenne, H., Burny, A., and Marbaix, G., Prog. Nucl. Acid Res. Mol. Biol., 1, 173 (1967).

    Article  Google Scholar 

  2. Fischer, S., Bruns, G. P., Lowy, B. A., and London, I. M., Proc. US Nat. Acad. Sci., 49, 219 (1963).

    Article  ADS  CAS  Google Scholar 

  3. Arnstein, H. R. V., Cox, R. A., and Hunt, J. A., Biochem. J., 92, 648 (1964).

    Article  CAS  Google Scholar 

  4. Kruh, J., Drefus, J. C., and Schapira, G., Biochim. Biophys. Acta, 91, 494 (1964).

    PubMed  CAS  Google Scholar 

  5. Weisberger, A. S., Wolfe, S., and Armentrout, S., J. Exp. Med., 120, 161 (1964).

    Article  CAS  Google Scholar 

  6. Hunt, J. A., and Wilkinson, B. R., Biochemistry, 6, 1688 (1967).

    Article  CAS  Google Scholar 

  7. Schaeffer, J., Favelukes, G., and Schweet, R., Biochim. Biophys. Acta, 80, 247 (1964).

    Google Scholar 

  8. Drach, J. C., and Lingrel, J. B., Biochim. Biophys. Acta, 129, 178 (1966).

    Article  CAS  Google Scholar 

  9. Brawerman, G., Bienzunski, N., and Eisenstadt, J., Biochim. Biophys. Acta, 103, 201 (1965).

    Article  CAS  Google Scholar 

  10. Nirenberg, M. W., and Matthaei, J. H., Proc. US Nat. Acad. Sci., 47, 1588 (1961).

    Article  ADS  CAS  Google Scholar 

  11. Clark, B. F. C., and Marcker, K. A., Nature, 211, 378 (1966).

    Article  ADS  CAS  Google Scholar 

  12. Adams, J. M., and Capecchi, M. R., Proc. US Nat. Acad. Sci., 55, 147 (1966).

    Article  ADS  CAS  Google Scholar 

  13. Webster, R. E., Engelhardt, D. L., and Zinder, N. D., Proc. US Nat. Acad. Sci., 55, 155 (1966).

    Article  ADS  CAS  Google Scholar 

  14. Lucas-Lenard, J., and Lipman, F., Proc. US Nat. Acad. Sci., 57, 1056 (1967).

    Article  ADS  Google Scholar 

  15. Hastings, J. R. B., and Kirby, K. S., Biochem. J., 100, 532 (1966).

    Article  CAS  Google Scholar 

  16. Savitsky, J. P., and Stand, F., Biochim. Biophys. Acta, 114, 419 (1966).

    Article  CAS  Google Scholar 

  17. Haenni, A. L., and Chapeville, F., Biochim. Biophys. Acta, 114, 135 (1966).

    Article  CAS  Google Scholar 

  18. Carbon, J. A., Biochem. Biophys. Res. Commun., 15, 1 (1964).

    Article  CAS  Google Scholar 

  19. Ozawa, H., and Sataka, K., J. Biochem., 42, 641 (1955).

    Article  CAS  Google Scholar 

  20. Capecchi, M. R., Proc. US Nat. Acad. Sci., 55, 1517 (1966).

    Article  ADS  CAS  Google Scholar 

  21. Schroeder, W. A., Jones, R. T., Cormick, J., and McCalla, K., Anal. Chem., 34, 1570 (1962).

    Article  CAS  Google Scholar 

  22. Dintzis, H. M., Proc. US Nat. Acad. Sci., 47, 247 (1961).

    Article  ADS  CAS  Google Scholar 

  23. DeWitt, W., and Ingram, V. M., Biochem. Biophys. Res. Commun., 27, 236 (1967).

    Article  CAS  Google Scholar 

  24. Rich, A., Eikenberry, E. F., and Malkin, L. I., Cold Spring Harbor Symp. Quant. Biol., 31, 303 (1966).

    Article  CAS  Google Scholar 

  25. Arnstein, H. R. V., and Rahamimoff, H., Nature, 219, 942 (1968).

    Article  ADS  CAS  Google Scholar 

  26. Clark, J. M., Chang, A. Y., Spiegelman, S., and Reichmann, M. E., Proc. US Nat. Acad. Sci., 54, 1193 (1965).

    Article  ADS  CAS  Google Scholar 

  27. Schwartz, J. H., J. Mol. Biol., 30, 309 (1967).

    PubMed  CAS  Google Scholar 

  28. Verhoef, N. J., Reinecke, C. J., and Bosch, L., Biochim. Biophys. Acta, 149, 305 (1967).

    Article  CAS  Google Scholar 

  29. Hunt, J. A., Biochem. J., 95, 541 (1965).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

LAYCOCK, D., HUNT, J. Synthesis of Rabbit Globin by a Bacterial Cell Free System. Nature 221, 1118–1122 (1969). https://doi.org/10.1038/2211118a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/2211118a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing