Abstract
ACETYLCHOLINE was first identified in animal tissue by Dale and Dudley1. They isolated acetylcholine as the double choline–acetylcholine dichloroplatinate from 40 kg of horse spleen. Its identity was demonstrated by elemental analysis and a mixed melting point determination with the appropriate reference compound. A large volume of pharmacological, chemical and physical evidence has since accumulated which indicates that acetylcholine is present in many tissues, especially neural tissue. Several other choline esters and related compounds have been found in extracts and homogenates of tissue from various animal species2–5.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Dale, H. H., and Dudley, H. W., J. Physiol., 68, 97 (1929).
Whittaker, V. P., Handbuch der Experimentellen Pharmakologie, 1–39 (Springer, Berlin, 1963).
Hosein, E. A., Proulx, P., and Ara, R., Biochem. J., 83, 341 (1962).
Hosein, E. A., and Koh, T. Y., Canad. J. Physiol. Pharmacol., 43, 657 (1965).
Bacq, Z. M., and Mazza, F. P., Arch. Intern. Physiol., 42, 43 (1935).
Stedman, E., and Stedman, E., Biochem. J., 31, 817 (1937).
Hosein, E. A., and Orzeck, A., Intern. J. Neuropharmacol., 3, 71 (1964).
Ryhage, R., Anal. Chem., 36, 759 (1964).
Jenden, D. J., and Hanin, I., Lamb, S. I., Anal. Chem., 40, 125 (1968).
Hanin, I., and Jenden, D. J., Biochem. Pharmacol. (in the press).
Hanin, I., thesis, Univ. California at Los Angeles (1968).
Hanin, I., Jenden, D. J., and Lamb, S. I., Proc. West. Pharmacol. Soc., 11, 144(1968).
Stavinoha, W. B., and Ryan, L. C., J. Pharmacol., 150, 231 (1965).
Stavinoha, W. B., Ryan, L. C., and Treat, E. L., Life Sci., 3, 689 (1964).
Hammar, C.-G., Holmstedt, B., and Ryhage, R., Anal. Biochem., 25 (1968).
Sweeley, C. C., Elliott, W. H., Fries, I., and Ryhage, R., Anal. Chem., 38, 549 (1966).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
HAMMAR, CG., HANIN, I., HOLMSTEDT, B. et al. Identification of Acetylcholine in Fresh Rat Brain by Combined Gas Chromatography–Mass Spectrometry. Nature 220, 915–917 (1968). https://doi.org/10.1038/220915a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/220915a0
This article is cited by
-
Spectroscopic Determination of Acetylcholine (ACh): A Representative Review
Topics in Current Chemistry (2023)
-
Acetylcholine in human placenta
Naunyn-Schmiedeberg's Archives of Pharmacology (1980)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
