Abstract
ANTIBODIES which are produced by developing cells can be detected and enumerated in the spleen of immunized animals either as plaque forming cells (PFC)1,2, or as rosette forming cells (RFC)3–5. In immunized mice the formation of plaques and rosettes has recently been shown to be attributable to two distinct populations of lymphoid cells6. Both PFC and RFC techniques also detect a “background” level of activity in the spleens of normal non-immunized animals. Using sheep red cells (SRC) as the test antigen, a background level of 0.05–0.5 PFC/106 splenocytes has been determined in non-immunized mice1,7. A much higher background level of 102–103 RFC/106 splenocytes8 has, however, been shown by the rosette method. This high background number of RFC could reflect (a) antibody forming cells; (b) cells passively coated with cytophilic antibody9; or (c) non-immunological activity.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Jerne, N. K., and Nordin, A. A., Science, 140, 405 (1963).
Ingraham, J. S., and Bussard, A., J. Exp. Med., 119, 667 (1964).
Nota, N. R., Liacopoulos-Briot, M., Stiffel, C., and Biozzi, G., CR Acad. Sci., 259, 1277 (1964).
Zaalberg, O. B., Nature, 202, 1231 (1964).
Cunningham, A. J., Nature, 207, 1106 (1965).
Zaalberg, O. B., Van der Meul, V. A., and Van Twisk, J. M., Nature, 210, 544 (1966).
Hege, J. S., and Cole, L. J., J. Immunol., 99, 61 (1967).
Biozzi, G., Stiffel, C., Mouton, D., Liacopoulos-Briot, M., Decreusefond, C., and Bouthiller, Y., Ann. Inst. Pasteur, 110, 7 (1966).
Boyden, S. V., and Sorkin, E., Immunology, 3, 272 (1960).
Baker, P. J., Bernstein, M., Pasanen, V., and Landy, M., J. Immunol., 97, 767 (1966).
Dent, P. B., and Good, R. A., Nature, 207, 491 (1965).
Boyden, S. V., Immunology, 7, 474 (1964).
Storb, U., and Weiser, R. S., J. Reticuloendothelial Soc., 4, 51 (1967).
Friedman, H., Experientia, 20, 564 (1964).
Nakano, M., and Braun, W., Science, 151, 338 (1966).
Schlezinger, M., J. Immunol.,, 93, 255 (1964).
Prindull, G., Zeit. Anat. und Entwickl., 125, 255 (1966).
Silverstein, A. M., Uhr, J. W., Kraner, K. L., and Lukes, R. J., J. Exp. Med., 117, 799 (1963).
La Via, M. F., Rowlands, D. T., and Black, M., Science, 140, 1219 (1963).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
LASKOV, R. Rosette Forming Cells in Non-immunized Mice. Nature 219, 973–975 (1968). https://doi.org/10.1038/219973a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/219973a0
This article is cited by
-
New developments about the association of SV40 with human mesothelioma
Oncogene (2003)
-
Natural lymphocytic rosettes in non-operated, thymectomized and bursectomized chickens
Experientia (1974)
-
Assay of antilymphocytic preparations by inhibition of erythrocyte rosette formation
Bulletin of Experimental Biology and Medicine (1971)
-
In vivo Specific Antigen Recognition by Rosette Forming Cells
Nature (1970)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
