Abstract
PREPARATION of mitochondria from Neurospora usually requires quite drastic treatments such as micro-mills, glass beads, sand and disruption of protoplasts. It also requires from 2 to 12 h after collection to isolate the mitochondrial fraction. We have developed a method for preparing mitochondria in 30 min using a combination of three new techniques—a nylon bag, a glass homogenizer and rapid centrifugation.
Similar content being viewed by others
Article PDF
References
Hall, D. O., and Greenawalt, J. W., Biochem. Biophys. Res. Commun., 17, 565 (1964).
Hall, D. O., and Greenawalt, J. W., J. Gen. Microbiol., 48, 419 (1967).
Nobel, P. S., Plant Physiol., 42, 1389 (1967).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
HALL, D., BALTSCHEFFSKY, H. Rapid Preparation of Neurospora Mitochondria. Nature 219, 968 (1968). https://doi.org/10.1038/219968a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/219968a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.