Abstract
N6-2′-O-DIBUTYRYL-3′5′(CYCLIC)-AMP (DCA) has a direct parathyroid hormone (PTH)-like action on bone explants in culture. It stimulates bone resorption, as manifested by the release of calcium, inorganic phosphate (Pi) and hydroxyproline from the explants; it enhances their production of lactate and of citrate; it increases synthesis and excretion of several lysosomal enzymes, acid p-nitro-phenylphosphatase, β-glucuronidase and N-acetyl-β-glucosaminidase. All these effects mimic those of PTH in this system1–4. Neither 3′5′(cyclic)-AMP (CA) or 5′-AMP, nor theophylline or caffeine, is active.
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VAES, G. Parathyroid Hormone-like Action of N6-2′-O-Dibutyryladenosine-3′5′ (Cyclic)-Monophosphate on Bone Explants in Tissue Culture. Nature 219, 939–940 (1968). https://doi.org/10.1038/219939a0
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DOI: https://doi.org/10.1038/219939a0
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