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LpI—Allotypic Determinant of Rabbit β-Lipoprotein

Nature volume 218, pages 595–596 (1968)Cite this article

Abstract

A “NEW” allotypic gene has been revealed on iso-immunization of eighteen rabbits with a single rabbit's serum during an attempt to produce anti-IgG allotypic antibodies (reviewed in ref. 1). The animals received fifteen intravenous injections, each consisting of 1 ml. of serum, biweekly (three courses of five injections, the first and second course followed by a month interval), and were bled a week after the last injection. None of the recipients formed iso-precipitins to rabbit IgG allotypic determinants, but one of them (rabbit No. 21) responded by producing anti-allotypic antibodies against rabbit serum β-lipoprotein. This antibody reacted well by double immunodiffusion test in agar-gel and by immunoelectrophoresis (see Fig, 1) using whole rabbit serum or the isolated fraction2,3 of β-lipoprotein as antigen. The electrophoretic mobility of purified β-lipoprotein fractions differed according to the protein concentration in the sample4. In addition, the precipitin lines stained positively for lipid.

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References

  1. Kelus, A. S., and Gell, P. G. H., Prog. Allergy, 11, 141 (1967).

    CAS  PubMed  Google Scholar 

  2. Stokes, R. P., J. Med. Lab. Tech., 23, 33 (1966).

    CAS  Google Scholar 

  3. Stokes, R. P., Jacobsson, A., and Walton, K. W., J. Atheroscler. Res., 7, 187 (1967).

    Article  CAS  Google Scholar 

  4. Walton, K. W., Immunochemistry, 1, 279 (1964).

    Article  CAS  Google Scholar 

  5. Kasukawa, R., Abeyounis, C. J., and Milgrom, F., Transplantation, 5, 1289 (1967).

    Article  CAS  Google Scholar 

  6. Berg, K., Series Haematologica, 1, 111 (1968).

    Google Scholar 

  7. Berg, K., Ceppellini, R., Curtoni, E. S., Mattiuz, P. L., and Bearn, A. G., I Congr. Internat. Soc. Transplant., 178 (Paris, 1967).

    Google Scholar 

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Authors and Affiliations

  1. Department of Experimental Pathology, University of Birmingham,

    A. S. KELUS

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  1. A. S. KELUS
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KELUS, A. LpI—Allotypic Determinant of Rabbit β-Lipoprotein. Nature 218, 595–596 (1968). https://doi.org/10.1038/218595a0

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