Abstract
WE have observed “unscheduled DNA synthesis” and non-conservative synthesis of DNA after the irradiation of mammalian cells with ultraviolet light1. We have presented autoradiographic evidence that unscheduled DNA synthesis occurred in HeLa cells after X-irradiation with 5,000 r.1. Normal semi-conservative DNA replication is considerable at this dose, so neutral equilibrium density gradient experiments to examine the molecular nature of this synthesis were precluded. In this communication we report that, after very large doses of X-rays to HeLa cells, incorporation of precursors into DNA occurs as a result of a process which has the characteristics of “repair-replication”, a phenomenon which has been correlated with enhanced survival in bacteria2.
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References
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PAINTER, R., CLEAVER, J. Repair Replication in HeLa Cells after Large Doses of X-irradiation. Nature 216, 369–370 (1967). https://doi.org/10.1038/216369a0
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DOI: https://doi.org/10.1038/216369a0
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