Abstract
ALVEOLAR cells are important because they are thought to produce surfactant1–3, a lipoprotein4–6 which lines the alveoli and is responsible for much of the stability of the pulmonary airspaces7. Previous work has shown that alveolar cells induced with M. bovis (B.C.G.) suspended in Hanks medium can incorporate leucine into protein and that such incorporation is inhibited (≥99 per cent) by puromycin. Extracellular radioactive protein appears during the incubations, which suggests either a non-specific release due to cell disruption, a more specific release dependent on energy or a combination of both factors8. This communication describes the release of radioactive protein into the suspending media by alveolar cells labelled with DL-leucine-l-14C and illustrates the depressive effect on this release of metabolic inhibitors.
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References
Klaus, M. H., Reiss, O. K., Tooley, W. H., Piel, C., and Clements, J. A., Science, 137, 750 (1962).
Buckingham, S., McNary, W. F., and Sommers, S. C., Science, 145, 1192 (1964)
Buckingham, S., Heineman, H. O., Sommers, S. C., and McNary, W. F., Amer. J. Path., 48, 1027 (1966).
Pattle, R. E., and Thomas, L. C., Nature, 189, 844 (1961).
Klaus, M. H., Clements, J. A., and Havel, R. J., Proc. US Nat. Acad. Sci., 142, 1858 (1961).
Fujiwara, T., Haruko, H., and Arakawa, T., Tohoku J. Exp. Med., 85, 33 (1965).
Pattle, R. E., Phys. Rev., 45, 48 (1965).
Massaro, D., Handler, A., and Bottoms, L., Amer. Rev. Resp. Dis. (in the press)
Cohn, Z. A., and Wiener, E., J. Exp. Med., 118, 991 (1963).
Heise, E. R., and Myrvick, Z. N., Fed. Proc., 25, 439 (1966).
Sutherland, E. W., Cori, C. F., Haynes, R., and Olsen, N. S., J. Biol. Chem., 180, 825 (1949).
Croxton, F. E., Elementary Statistics with Applications in Medicine and the Biological Sciences (Dover, New York, 1959).
Schneider, W. C., in Methods in Enzymology (edit. by Colowick, S. P., and Kaplan, N. O.), 3, 680 (Academic Press, New York, 1956).
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MASSARO, D. Synthesis of Proteins by Alveolar Cells. Nature 215, 646–647 (1967). https://doi.org/10.1038/215646a0
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DOI: https://doi.org/10.1038/215646a0
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