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In vitro Synthesis of Bacteriophage Lysozyme

Abstract

Active lysozyme of bacteriophage T4 has been synthesized in a cell free system programmed by RNA from cells infected with the phage. The appearance of this lysozyme activity has the characteristics of de novo synthesis.

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References

  1. Nathans, D., Notani, G., Schwartz, J. H., and Zinder, N. D., Proc. US Nat. Acad. Sci., 48, 1424 (1962).

    Article  ADS  CAS  Google Scholar 

  2. Nathans, D., J. Mol. Biol., 13, 521 (1965).

    Article  CAS  Google Scholar 

  3. Capecchi, M., J. Mol. Biol., 21, 173 (1966).

    Article  CAS  Google Scholar 

  4. Capecchi, M. R., and Gussin, G. N., Science, 149, 417 (1965).

    Article  ADS  CAS  Google Scholar 

  5. DeVries, J. K., and Zubay, G., Proc. US Nat. Acad. Sci., 57, 1010 (1967).

    Article  ADS  CAS  Google Scholar 

  6. Imai, M., Yura, T., and Marushige, K., Biochem. Biophys. Res. Commun., 11, 270 (1963).

    Article  CAS  Google Scholar 

  7. Sueoka, N., and Kano-Sueoka, T., Proc. US Nat. Acad. Sci., 52, 1535 (1964).

    Article  ADS  CAS  Google Scholar 

  8. Nirenberg, M., Caskey, T., Marshall, R., Brimacombe, R., Kellogg, D., Doctor, B., Hatfield, D., Levin, J., Rottman, F., Pestka, S., Wilcox, M., and Anderson, F., Cold Spring Harbor Symp. Symp. Quant. Biol., 31, 11 (1966).

    Article  CAS  Google Scholar 

  9. Waters, L. C., and Novelli, G. D., Proc. US Nat. Acad. Sci., 57, 979 (1967).

    Article  ADS  CAS  Google Scholar 

  10. Neidhardt, F. C., and Earhart, C. F., Cold Spring Harbor Symp. Quart Biol., 31, 557 (1966).

    Article  CAS  Google Scholar 

  11. Inouye, M., and Tsugita, A., J. Mol. Biol., 22, 193 (1966).

    Article  CAS  Google Scholar 

  12. Gesteland, R. F., J. Mol. Biol., 16, 67 (1966).

    Article  CAS  Google Scholar 

  13. Nirenberg, M. W., and Matthaei, J. H., Proc. US Nat. Acad. Sci., 47, 1588 (1961).

    Article  ADS  CAS  Google Scholar 

  14. Gesteland, R. F., and Boedtker, H., J. Mol. Biol., 8, 496 (1964).

    Article  CAS  Google Scholar 

  15. Nygaard, A. P., and Hall, B. D., J. Mol. Biol., 9, 125 (1964).

    Article  CAS  Google Scholar 

  16. Sekiguchi, M., and Cohen, S. S., J. Mol. Biol., 8, 638 (1964).

    Article  CAS  Google Scholar 

  17. Brenner, S., Biochim. Biophys. Acta, 18, 531 (1955).

    Article  CAS  Google Scholar 

  18. Szer, W., and Ochoa, S., J. Mol. Biol., 8, 823 (1964).

    Article  CAS  Google Scholar 

  19. Nakamoto, T., and Kolakofsky, D., Proc. US Nat. Acad. Sci., 55, 606 (1966).

    Article  ADS  CAS  Google Scholar 

  20. Thach, R. E., Sundararajan, A., Dewey, K. F., Brown, J. C., and Doty, P., Cold Spring Harbor Symp. Quant. Biol., 31, 85 (1966).

    Article  CAS  Google Scholar 

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SALSER, W., GESTELAND, R. & BOLLE, A. In vitro Synthesis of Bacteriophage Lysozyme. Nature 215, 588–591 (1967). https://doi.org/10.1038/215588a0

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