Abstract
THE electron microscope particle-counting technique of Backus and Williams1,2, which involves the use of shadow-cast specimens, has been generally favoured in investigations of viruses involving particle counting. Watson3 modified the method by using phosphotungstate negative staining4,5 to reveal the virus particles in more structural detail and to make identification easier. The modification facilitates counting of virus particles in relatively impure preparations.
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References
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GÁMEZ, R., BLACK, L. Application of Particle-counting to a Leafhopper-borne Virus. Nature 215, 173–174 (1967). https://doi.org/10.1038/215173a0
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DOI: https://doi.org/10.1038/215173a0
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