Allograft Survival prolonged by Heterologous Spleen Cells in ‘Millipore’ Diffusion Chambers

Abstract

WE have mixed heat-inactivated lymphoid cells from C57Bl mice with rat spleen cells. The mixture was placed in ‘Millipore’ diffusion chambers which were introduced intraperitoneally into C57Bl mice. Five days later these animals were grafted with CBA skin. We hoped that the rat cells would produce antibody against antigenic moieties present in the mouse lymphoid cells, and that the antibody would diffuse out of the chambers, inactivate host lymphoid cells and thus depress the immune response of the treated animal. Previous workers^1,2, employing a rather similar experimental model, have shown that lymphoid cells mixed in diffusion chambers with sheep red cells can produce antibody against the latter. The ability of a heterologous anti-lymphocyte serum to prolong allograft survival is now well established^3–6.