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Isolation of Half Molecules of DNA from Gram-negative Bacteria

Nature volume 215, pages 1185–1186 (1967)Cite this article

Abstract

THE isolation of intact DNA of the same size and form as that present in the organism is possible in the case of viruses1 but presents problems in bacteria and higher organisms, principally as a result of the action of degrading enzymes and mechanical shear. Isolated bacterial DNA usually has a relatively low molecular weight (S°20,w between 11–33)2,3 except in the cases of E. coli4, B. subtilis4 (S°20,w of 81) and H. influenzae5 (S°20,w of 94).

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Author notes

  1. M. J. PITOUT

    Present address: N.N.R.I., P.O. Box 395, Pretoria, South Africa

Authors and Affiliations

  1. Department of Microbiology, University of Pretoria,

    M. J. PITOUT & I. J. MARÉ

Authors
  1. M. J. PITOUT
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  2. I. J. MARÉ
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PITOUT, M., MARÉ, I. Isolation of Half Molecules of DNA from Gram-negative Bacteria. Nature 215, 1185–1186 (1967). https://doi.org/10.1038/2151185a0

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