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DNA and RNA Synthesis and the Formation of Blastogenic Factor in Mixed Leucocyte Cultures

Nature volume 215, pages 80–81 (1967)Cite this article

Abstract

AFTER incubation for several days, cultures of mixed leucocytes from two individuals contain immature mononuclear cells. These cells incorporate thymidine into DNA (refs. 1 and 2). A material which stimulates blastogenesis of allogeneic leucocytes is released into the surrounding medium when human leucocytes are cultured in vitro3–5. More of this material is released into the medium from mixed leucocyte cultures than from cultures of leucocytes from single donors. This communication reports experiments which were designed to determine DNA and RNA synthesis and the formation of blastogenic factor in mixed leucocyte cultures after various periods of incubation.

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Authors and Affiliations

  1. Division of Haematology, Department of Medicine, Royal Victoria Hospital and McGill University Clinic, Montreal, Canada

    SHINPEI KASAKURA & LOUIS LOWENSTEIN

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  1. SHINPEI KASAKURA
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  2. LOUIS LOWENSTEIN
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KASAKURA, S., LOWENSTEIN, L. DNA and RNA Synthesis and the Formation of Blastogenic Factor in Mixed Leucocyte Cultures. Nature 215, 80–81 (1967). https://doi.org/10.1038/215080a0

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  • DOI: https://doi.org/10.1038/215080a0

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