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Electron Transport Carriers in Plant Mitochondria

Abstract

THE extent of reduction of the electron transport carriers in tightly coupled higher plant mitochondria has been measured during various mitochondrial steady state conditions. The mitochondria used for the experiments reported here were extracted from potato tubers (Solanum tuberosum, var. Katadin) and from the hypocotyls of aetiolated black valentine bean seedlings (Phaseolus vulgaris). The method of mitochondrial preparation, described previously1, produces uniform suspensions of mitochondria which are remarkably free of contaminating sub-cellular structures2. These mitochondria oxidize, with tight adenosine diphosphate (ADP) control, succinate, malate and externally added reduced nicotinamide-adenine dinucleotide (NADH). The electron transport carriers of the higher plant mitochondria include pyridine nucleotides (PN), flavoproteins (FP), three b cytochromes (b556, b560, b565), two c cytochromes (c550, c552), and cytochrome oxidase (cytochromes a + a3) (ref. 3).

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References

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BONNER, W., PLESNICAR, M. Electron Transport Carriers in Plant Mitochondria. Nature 214, 616–617 (1967). https://doi.org/10.1038/214616a0

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