Abstract
WHEN lymphoid cells from animals producing haemolytic antibody are incubated with target erythrocytes in a semi-solid agar medium, antibody diffuses from the cells containing it and becomes attached to the red cells. If complement is added after this incubation, the presence of antibody is shown by the formation of plaques of haemolysis1,2. Killing the cells before incorporating them in agar, or adding metabolic inhibitors such as cyanide2 to the agar before incubation, prevents plaque formation. This has been taken as evidence that the antibody-forming cells in the agar are alive and actively secreting antibody. If this were so it would be possible to investigate the modes of action of some immunosuppressive agents by observing their effects when added to the agar medium.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Jerne, N. K., and Nordin, A. A., Science, 140, 405 (1963).
Jerne, N. K., Nordin, A. A., and Henry, C., in Cell-bound Antibodies (edit. by Amos, B., and Koprowski, H.), 109 (Wistar Institute Press, Philadelphia, 1963).
Cecil, R., and McPhee, J. R., Adv. Protein Chem., 14, 255 (1959).
Catsimpoolas, N., and Wood, J. L., J. Biol. Chem., 239, 4132 (1964).
Wood, J. L., and Catsimpoolas, N., J. Biol. Chem., 238, 2887 (1963).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
BERENBAUM, M. Effect of some Metabolic Inhibitors on Plaque Formation in Jerne Plates. Nature 214, 590–591 (1967). https://doi.org/10.1038/214590a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/214590a0
This article is cited by
-
Use of 2,4-dinitrophenol for immunosuppressive action on the recipient during implantation of fetal organs in mice
Bulletin of Experimental Biology and Medicine (1992)
-
Cell Respiration as a Requirement for Antibody Release in vitro
Nature (1968)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
