Abstract
ACTIVITY of lactic dehydrogenase (L-lactate: NAD+ oxidoreductase, E.C, 1.1.1.27) has been determined in the preimplantation mouse embryo1. The one-cell mouse embryo had a lactic dehydrogenase (LDH) activity nine times greater than the most active adult mouse tissue. This finding, in addition to several other observations, indicates the importance of this enzyme to the early mouse embryo and perhaps to all mammalian embryos. Culture work has shown that the two-cell mouse embryo can develop into a blastocyst in vitro when pyruvate or lactate is present in the medium as the only energy source but not when glucose alone is present2. The optimum concentration of lactate in the medium is 5 × 10−2 moles/l, compared with 5×10−4 moles/l, for pyruvate3. The concentration of lactate is large in the Fallopian tube of the rabbit during the first 3 days after ovulation4.
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BRINSTER, R. Lactic Dehydrogenase Activity in Preimplantation Rat Embryo. Nature 214, 1246–1247 (1967). https://doi.org/10.1038/2141246a0
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DOI: https://doi.org/10.1038/2141246a0
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