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Spontaneous Mutants of Yeast Deficient in Glycogen

Abstract

AN unusual type of spontaneous mutation has been detected in some diploid strains of Saccharomyces cerevisiae. Iodine staining of yeast colonies has revealed the presence of a high proportion of glycogen deficient mutants. In yeasts, glycogen is a reserve material, and the impaired storage ability, together with the high frequency of occurrence, made these mutants especially interesting. The iodine staining test has also shown that respiration deficient mutants growing on nutrient agar are deficient in glycogen when compared with the wild type yeast. The two types of mutation can be easily distinguished.

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CHESTER, V. Spontaneous Mutants of Yeast Deficient in Glycogen. Nature 214, 1237–1238 (1967). https://doi.org/10.1038/2141237a0

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