Abstract
THE term “endotoxin” is used to denote the lipopolysaccharides of high molecular weight (1 million or above) which are considered to be integral structural units of the cell walls of Gram negative bacteria1. The conventional method of preparing endotoxin is by chemical extraction from intact cells (or cell walls) using, for instance, trichloroacetic acid at 4° C (ref. 2), 45 per cent aqueous phenol at 65°–68° C (ref. 3), or mixtures of ether and water at 6°–12° C (ref. 4). Heterogeneous extracts are obtained which may require extensive purification before they yield the final homogeneous product5.
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CRUTCHLEY, M., MARSH, D. & CAMERON, J. Free Endotoxin. Nature 214, 1052 (1967). https://doi.org/10.1038/2141052a0
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DOI: https://doi.org/10.1038/2141052a0
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