Abstract
EARLIER we reported that the net electrical charge of rabbit antibodies of the immunoglobulin G class to various natural and synthetic antigens was inversely related to the net electrical charge of the antigens1. Thus, under a certain set of conditions, antibodies to acidic antigens were found to predominate in the first chromatographic fraction eluted from diethylaminoethyl-‘Sephadex’, whereas antibodies to basic antigens were mostly in the second fraction. Immunoelectrophoresis revealed that at pH 8.6, the second fraction moved faster towards the anode than the first fraction. This effect was shown to be independent of the immunological specificity of the IgG antibodies investigated, as the electrophoretic mobility of anti-dinitrophenyl (DNP) antibodies elicited by DNP conjugates of various acidic and basic proteins and synthetic polypeptides was related to the net charge of the macromolecular carrier.
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ROBBINS, J., MOZES, E., RIMON, A. et al. Correlation between Net Charge of Antigens and Electrophoretic Mobility of Immunoglobulin M Antibodies. Nature 213, 1013–1014 (1967). https://doi.org/10.1038/2131013a0
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DOI: https://doi.org/10.1038/2131013a0
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