Abstract
METHODS which use enzymes for the analysis of steroid hormones deserve attention because they have greater specificity than chemical and physico-chemical methods. The low sensitivity and low degree of accuracy of current methods for the determination of coenzymes, however, have limited the use of enzymes for the determination of those steroid hormones that occur in low concentrations in tissues and body fluids such as blood. This communication reports a solution to this problem, and it uses an enzyme cycling procedure to increase sensitivity, and the measurement of labelled steroid to increase accuracy.
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References
Marcus, P. I., and Talalay, P., J. Biol. Chem., 218, 661 (1956).
Lowry, O. H., Passonneau, J. V., Schulz, D. W., and Rock, M. K., J. Biol. Chem., 236, 2746 (1961).
Delin, S., Squire, P. G., and Porath, J., Biochim. Biophys. Acta, 89, 398 (1964).
Bush, I. E., Biochem. J., 50, 370 (1951).
Brownie, A. C., van der Molen, H. J., Nishizawa, E.E., and Eik-Nes, K. B., J. Clin. Endocrinol. Metab., 24, 1091 (1964).
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CARSTENSEN, H. Ultramicro Enzyme Analysis of Testosterone. Nature 212, 1604–1605 (1966). https://doi.org/10.1038/2121604a0
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DOI: https://doi.org/10.1038/2121604a0
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