Cell Culture of Fibroblasts from Clupea harengus Gonads

Abstract

RECENT advances in animal cell culture have been largely restricted to warm-blooded vertebrates, particularly mammals. Cells of fishes have been given limited attention in culture investigations, although they include the majority of species in the vertebrate fauna. Wolf et al.¹ were the first to prepare cultures of fish cells using tryp-sinization and commercial mammalian media, but they incubated their cultures at 19° C rather than at the higher mammalian cell incubation temperature. They cultured cells of several freshwater species and Wolf later established a cell line with rainbow trout cells². Roberts^3,4 prepared cell cultures of several freshwater centrarchids for chromosome study.