Abstract
DICOTYLEDONS grown on White's medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) readily produce callus which is an ideal substrate for plant nematodes1,2. Monocotyledons produce callus less readily. Morel and Wetmore3 initiated callus in species of Amorphophallus with carefully balanced vitamins and added naphthalene-acetic acid (NAA), and maintained the callus by the addition of coconut milk. Carew and Schwarting4 obtained callus from the embryo of rye by adding yeast extract, casein hydrolysate and 2,4-D to the medium. The production of oat and onion callus tissues is described below. The oat callus was used to culture the chrysanthemum nematode, Aphelenchoides ritzemabosi (Schwartz).
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01 March 1967
In the communication, entitled "Production of Oat Callus and its Susceptibility to a Plant Parasitic JSTematode" by Dr. J. M. Webster (Nature, 212, 1472; 1966), the third sentence in the second paragraph should read "Glucose (20 gm/1.) or the same quantity of sucrose was used . . .".
References
Krusberg, L. R., Nematologica, 6, 181 (1961).
Webster, J. M., and Lowe, D., Parasitology, 56, 313 (1966).
Morel, G., and Wetmore, R. H., Amer. J. Bot., 38, 138 (1951).
Carew, D. P., and Schwarting, A. E., Bot. Gaz., 119, 237 (1958).
Webster, J. M., Plant. Pathol. (in the press, 1967).
Thomas, E. W., Loughman, B. C., and Powell, R. G., Nature, 204, 286 (1964).
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WEBSTER, J. Production of Oat Callus and its Susceptibility to a Plant Parasitic Nematode. Nature 212, 1472 (1966). https://doi.org/10.1038/2121472a0
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DOI: https://doi.org/10.1038/2121472a0
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