Abstract
THERE is as yet no ideal method for demonstrating pleuropneumonia-like organisms (PPLO) in tissue culture. Isolation of an infecting strain, particularly if this is intracellular, is frequently technically difficult and is always time-consuming. Cytological examination of monolayer cultures for evidence of PPLO infection is rapid, but staining artefacts may give rise to difficulty in interpretation. Indirect evidence of infection by transmission to susceptible ‘indicator cultures’1 is only valid if these can be maintained free from accidental contamination by PPLO.
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SHEDDEN, W., COLE, B. Rapid Method for demonstrating Intracellular Pleuropneumonia-like Organisms in a Strain of Hamster Kidney Cells (BHK 21 CI3). Nature 210, 868 (1966). https://doi.org/10.1038/210868a0
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DOI: https://doi.org/10.1038/210868a0
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