Abstract
RECENTLY we reported1 the separation of pinguinain, the enzyme present in the fruit of the tropical plant Bromelia pinguin L. Using the technique of gel-nitration, we isolated the enzyme from the fruit juice and from crude acetone precipitates which also contained carbohydrates and low molecular weight peptides. Further work on this enzyme led to its crystallization, the procedure for which is the object of this communication.
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References
Toro-Goyco, E., and Matos, M., Nature, 203, 82 (1964).
Toro-Goyco, E., Matos, M., and Cancio, M., Fed. Proc., 22, 528 (1963).
Mills, G. L., in Chromatographic and Electrophoretic Techniques, edit. by Smith, I., 1, 143 (Interscience Publishers, Inc., New York, 1960).
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TORO-GOYCO, E., MATOS, M. Pinguinain : a Simple Method for its Crystallization. Nature 210, 527–529 (1966). https://doi.org/10.1038/210527b0
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DOI: https://doi.org/10.1038/210527b0
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