Abstract
THE isolation of polyribosomes from rat liver has been described in previous communications1–4. In addition to the polyribosomes found in the 75,000g pellet, three coloured layers localized in the interface between 1.5 M and 2 M sucrose can be isolated. The lower layer consists mainly of rough endoplasmie reticulum (membranes with attached ribosomes)2. In the upper layers the membranes are almost devoid of ribosomes. In both these layers polyribosomes are frequently encountered which are connected to the membrane surface only by way of one single terminal monomer (compare Fig. 1E).
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References
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BENEDETTI, E., BONT, W. & BLOEMENDAL, H. Structural Aspects of Polyribosomes and Endoplasmie Reticulum Fragments isolated from Rat Liver. Nature 210, 1156–1157 (1966). https://doi.org/10.1038/2101156a0
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DOI: https://doi.org/10.1038/2101156a0
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