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Electrophoretic Separation of Two Plaque-type Variants of Encephalomyocarditis Virus

Nature volume 210, pages 1080–1081 (1966)Cite this article

Abstract

THE surface properties of large (r) and small (r+) (formerly designated l and s respectively (ref. 1)) plaque-forming encephalomyocarditis (EMC) virus1 have been compared using a sucrose-gradient electrophoretic technique2. A difference was suspected because the relative plaque sizes changed on the addition of polyanions or polycations to the agar overlay of the plaque-assay1,3. Both variants produced ‘small’ plaques in the presence of excess polyanions (for example, sulphated polysaccharides), and ‘large’ plaques in the presence of excess polycations (such as diethylaminoethyl-dextran)1.

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References

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Author notes

  1. DAVID C. BREEZE

    Present address: St. Jude Hospital, P.O. Box 318, Memphis, Tennessee

Authors and Affiliations

  1. Institute of Virology, University of Glasgow,

    DAVID C. BREEZE & H. V. THORNE

Authors
  1. DAVID C. BREEZE
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  2. H. V. THORNE
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BREEZE, D., THORNE, H. Electrophoretic Separation of Two Plaque-type Variants of Encephalomyocarditis Virus. Nature 210, 1080–1081 (1966). https://doi.org/10.1038/2101080a0

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