Abstract
IN a recent paper1, Dr. B. D. Lake states that an important proportion of the enzyme activity revealed by substrate film methods may be attributed to the non-specific action of proteolytic enzymes dissolving the gelatine constituent of the film. Such a statement leaves the impression that the substrate film methods for RNase and DNase are unreliable and that results obtained so far by these methods have been erroneously interpreted.
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Lake, B. D., Nature, 209, 521 (1966).
Sierakowska, H., and Shugar, D., Acta Biochim. Polon., 8, 427 (1961).
Daoust, R., Intern. Rev. Cytol., 18, 191 (1965).
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DAOUST, R. Demonstration of Ribonuclease and Deoxyribonuclease Activities by Substrate Film Methods. Nature 210, 1077 (1966). https://doi.org/10.1038/2101077a0
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DOI: https://doi.org/10.1038/2101077a0
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