Abstract
DURING the course of histochemical investigations into various metabolic disorders, the substrate-film methods for the demonstration of ribonuclease and deoxyribonuclease1,2 were examined. Satisfactory thin films were obtained by dipping chemically cleaned slides into the DNA or RNA gelatine mixtures at 60° C and then shaking off the excess liquid. The films were allowed to stand vertically at 60° C for 5 min before wiping clean the bottom quarter of the slide. This procedure gave stable films which did not lift or distort during the subsequent operations of fixation in formalin, incubation and staining. The gelatine was obtained from B.D.H. (‘Gold Label’ grade), and the DNA and UNA from L. Light.
Similar content being viewed by others
Article PDF
References
Daoust, R., Exp. Cell Res., 12, 203 (1957).
Daoust, R., and Amano, H., J. Histochem. Cytochem., 8, 131 (1960).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
LAKE, B. Ribonuclease and Deoxyribonuclease Substrate Film Methods : a Pitfall in the Interpretation of Results. Nature 209, 521 (1966). https://doi.org/10.1038/209521a0
Issue Date:
DOI: https://doi.org/10.1038/209521a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.