Autoradiographic Electrophoresis of Tritium-labelled Compounds in a Thin Layer of Buffered Photographic Gelatine

Abstract

GEL electrophoresis allows the detection of radioactive compounds by contact with a photographic film. I have tried to separate radioactive ions by electrophoresis, using a thin layer of radiosensitive photographic gelatin as supporting medium. I hoped thus to locate smaller amounts of radioactive substances within a shorter time and, above all, to achieve higher efficiency in detection and location of tritium-labelled molecules through autoradiography. This communication gives a brief account of the first runs of autoradiographic electrophoresis in a thin layer of buffered photographic gelatine and affords a glimpse of the possibilities of this new type of supporting medium.